Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606172804_xu7i GGGAGTCTGAGTTCCTCTCCTGATGTCTAGGG(+AAAAAA)GTACCAGGTTCGCATGTAGTACAAACACATG NGG 10 8 17 8 24 0 100 5 10
    240606172802_ehk9 ACCTCCATGTGTTTGTACTACATGCGAACCTG(+AAAAAA)GTACCCCTAGACATCAGGAGAGGAACTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606172800_pfjw CTCTGTGTTCTCTGACTTTAGAAGTTGGCCGG(+AAAAAA)GATCCAAGCGTAGAGACAAGTGAAACTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606172758_kwsm TTAAAATCAAGTTTCACTTGTCTCTACGCTTG(+AAAAAA)GATCCCGGCCAACTTCTAAAGTCAGAGAACA NGG 10 8 17 8 24 0 100 5 10
    240606172757_avpe GGATGATTTATGGTCTCAACTGCAACACTGAG(+AAAAAA)GATCCAAGTGACACTCCAGTGATTGCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606172756_j45g TAAAATCAGGGCAATCACTGGAGTGTCACTTG(+AAAAAA)GATCCTCAGTGTTGCAGTTGAGACCATAAAT NGG 10 8 17 8 24 0 100 5 10
    240606172754_1h3n TAAAAAAAAAACCACCTTTTTATTGAACACAG(+AAAAAA)GATCCCCAAAGGCCACATATCAAAATATACA NGG 10 8 17 8 24 0 100 5 10
    240606172752_2yjw TTACATGTATATTTTGATATGTGGCCTTTGGG(+AAAAAA)GATCCTGTGTTCAATAAAAAGGTGGTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240606172749_yhu2 GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+AAAAAA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606172748_x9in GCAAACAGCAAGAGGCTTTATTGGGAACACGG(+AAAAAA)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10