Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174621_6pbl TTGTACAGCTGCGAGGGAGGGCACAGATGCAG(+AAAAAA)GTACCTGCGCACTCCGCCCTTCTCTCTTACA NGG 10 8 17 8 24 0 100 5 10
    240606174620_9fx1 GGGTGTGTAAGAGAGAAGGGCGGAGTGCGCAG(+AAAAAA)GTACCTGCATCTGTGCCCTCCCTCGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606174617_y3y2 TTCTAGGCACACTCCTTGCAGTAGGCTCCAGG(+AAAAAA)GTACCTTGGAACTGCAACCAAGGCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606174615_cf7f ATTCCTTACCATGCCTTGGTTGCAGTTCCAAG(+AAAAAA)GTACCCTGGAGCCTACTGCAAGGAGTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240606174614_1mke GTCTTCTCTATTTGTCTATTAATTGATATCTG(+AAAAAA)GATCCCCATGAAATTTTGAGAAAGCTACAGA NGG 10 8 17 8 24 0 100 5 10
    240606174612_3lq0 GCAATTCTGTAGCTTTCTCAAAATTTCATGGG(+AAAAAA)GATCCAGATATCAATTAATAGACAAATAGAG NGG 10 8 17 8 24 0 100 5 10
    240606174611_3zwe TTCCCTAAGGGAACTTTGAAGTTCATGTGTTG(+AAAAAA)GATCCTTCAACACATGGTAAGCACTGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606174610_slvx GCTCCACCTGCAGTGCTTACCATGTGTTGAAG(+AAAAAA)GATCCAACACATGAACTTCAAAGTTCCCTTA NGG 10 8 17 8 24 0 100 5 10
    240606174607_6a7h ACATGGAAAAGGACAAAGAGACCATACCTGGG(+AAAAAA)GATCCATCCCATTTACAGCCACTAAACCTGG NGG 10 8 17 8 24 0 100 5 10
    240606174606_0syd AGCATCCAGGTTTAGTGGCTGTAAATGGGATG(+AAAAAA)GATCCCCAGGTATGGTCTCTTTGTCCTTTTC NGG 10 8 17 8 24 0 100 5 10