Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171745_ojpg CTTTTCTTGATTGATGTAGCTATCTTCGGGTG(+AAAAAA)GTACCATCCCTAGGCAGGTAGGTCTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606171744_i4t2 AGTCAGAAAGAACTCCATCACGAGGTAAGGAG(+AAAAAA)GTACCGTGTCCAGGAGCTTGCTCCACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606171742_78si TTTGGGAACTGTGGAGCAAGCTCCTGGACACG(+AAAAAA)GTACCTCCTTACCTCGTGATGGAGTTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606171741_31k6 AGACAATCTCCGAAGCAAGAATGGTTTATTCG(+AAAAAA)GATCCTTGTTCAGTTATGTTTTGAGCTGACT NGG 10 8 17 8 24 0 100 5 10
    240606171740_nq72 CCAAAAGTCAGCTCAAAACATAACTGAACAAG(+AAAAAA)GATCCGAATAAACCATTCTTGCTTCGGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606171739_0926 GCCTCCGTATCCCCTGGAAATGTTTTCAGATG(+AAAAAA)GATCCCTTGGGTTCTCTGCTACCTAGTAATC NGG 10 8 17 8 24 0 100 5 10
    240606171736_nfbi CTGGAGATTACTAGGTAGCAGAGAACCCAAGG(+AAAAAA)GATCCATCTGAAAACATTTCCAGGGGATACG NGG 10 8 17 8 24 0 100 5 10
    240606171735_wxf2 GCCATCGGTGCGGTGCGGTGCGTGGATGCCTG(+AAAAAA)GATCCCAGCAGCAGCAGACATGGGGCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606171733_4iqc GTTTGCACCTGCCCCATGTCTGCTGCTGCTGG(+AAAAAA)GATCCAGGCATCCACGCACCGCACCGCACCG NGG 10 8 17 8 24 0 100 5 10
    240606171731_zwgs ACCACATGGTGGCCCACAACCATATGTAATGG(+AAAAAA)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10