Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171543_uspv ACGTGGGAAAGCGTCACAGGTGGCATCCGAAG(+AAAAAA)GATCCTTCTGCCATGTGTTGTGCATCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606171541_j3tg CCCTGGCTGAGATGCACAACACATGGCAGAAG(+AAAAAA)GATCCTTCGGATGCCACCTGTGACGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606171540_5lrl CAAAGTAGGACATTAGCCTTCAGCAAGTTTGG(+AAAAAA)GTACCTAAAAGTTTTGTCCTTGTTTTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240606171539_eyzu AAATCTAGAGAAAACAAGGACAAAACTTTTAG(+AAAAAA)GTACCCAAACTTGCTGAAGGCTAATGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240606171537_tebd GATGTGGAGGCAAGACATGGGTAAAGATTCAG(+AAAAAA)GATCCCTCCCTCCCTCAGTAAATGGTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240606171535_7y87 CAAGGTTGAAACCATTTACTGAGGGAGGGAGG(+AAAAAA)GATCCTGAATCTTTACCCATGTCTTGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606171534_obht TTGGTGACTCTGAAGACCAGAAAAGCGTGTTG(+AAAAAA)GATCCCAAGAACCAGAGTTGTAGGTGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606171533_pihn GGCTCACAACCACCTACAACTCTGGTTCTTGG(+AAAAAA)GATCCAACACGCTTTTCTGGTCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240606171531_692d GGGTGGCTTTAGATTTGCTTATTAGCTGAAGG(+AAAAAA)GATCCCTCCTGCTTCTGGGATTACAGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606171530_yver CAGCAGAGGACTGTAATCCCAGAAGCAGGAGG(+AAAAAA)GATCCCTTCAGCTAATAAGCAAATCTAAAGC NGG 10 8 17 8 24 0 100 5 10