Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171512_8125 GCATATGAGAGCAAGCTGTCAGCAAGGGGCTG(+AAAAAA)GTACCTGTCCCTACAAGTTGGCAATAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606171510_w04o CATCACCAGCTATTGCCAACTTGTAGGGACAG(+AAAAAA)GTACCAGCCCCTTGCTGACAGCTTGCTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606171508_esq5 GAAGGCAGGGAGGAGTGGCTTAGACAACCGAG(+AAAAAA)GATCCAGTTCCCAGGGTCCAGACGGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606171507_o6ln GGTTGAGCCAGCCGTCTGGACCCTGGGAACTG(+AAAAAA)GATCCTCGGTTGTCTAAGCCACTCCTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606171505_pgyd GGTCTAATGATGAGCAGATGGTAATGTATGGG(+AAAAAA)GTACCCTTGTGGCTCGCCTTTGGTGGGTACT NGG 10 8 17 8 24 0 100 5 10
    240606171503_crrb GTTTCAGTACCCACCAAAGGCGAGCCACAAGG(+AAAAAA)GTACCCCATACATTACCATCTGCTCATCATT NGG 10 8 17 8 24 0 100 5 10
    240606171502_5twx GCCATCGTCTCTACACAGGAGGCCGTTTCTAG(+AAAAAA)GATCCCAGCCTGCTCAGCGAGAGGTCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606171500_33gh CATTTGCTCTGACCTCTCGCTGAGCAGGCTGG(+AAAAAA)GATCCTAGAAACGGCCTCCTGTGTAGAGACG NGG 10 8 17 8 24 0 100 5 10
    240606171459_8fdc GCATGCAGGCCTTCCAGGAGGAACACACGTGG(+AAAAAA)GTACCTAGAAACCTAGTGAGGAATGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606171458_q3qm TCTAAACTTCCCATTCCTCACTAGGTTTCTAG(+AAAAAA)GTACCCACGTGTGTTCCTCCTGGAAGGCCTG NGG 10 8 17 8 24 0 100 5 10