Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171410_w315 TGACTAAACTATCTATCATTGACACAACTGGG(+AAAAAA)GATCCTCCAGGAGCACGGGTCAGTCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606171409_act9 ATTCTGTCCTTGACTGACCCGTGCTCCTGGAG(+AAAAAA)GATCCCCAGTTGTGTCAATGATAGATAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606171407_hl9r CTCTGCATCTGGCTTTTTACATGGCTGCTGGG(+AAAAAA)GATCCAAACTCGGGATATCATGCTTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240606171406_w0mr GGCCGTGGTACAAGCATGATATCCCGAGTTTG(+AAAAAA)GATCCCCAGCAGCCATGTAAAAAGCCAGATG NGG 10 8 17 8 24 0 100 5 10
    240606171404_orm2 TACTGCAAGAAAAAAAAATAAAACATTTCTTG(+AAAAAA)GTACCATAACTGTATCATTGTACCCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240606171403_jjco CCCTAGCATTTGGGTACAATGATACAGTTATG(+AAAAAA)GTACCAAGAAATGTTTTATTTTTTTTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240606171400_b77x TTCCCTGCATGCCTCTTCCCCTGAGCCCAGGG(+AAAAAA)GTACCTGGTCCCCATTTCCTACTCCTCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606171358_3g0h TTGTTAAAAGAGGAGTAGGAAATGGGGACCAG(+AAAAAA)GTACCCCTGGGCTCAGGGGAAGAGGCATGCA NGG 10 8 17 8 24 0 100 5 10
    240606171356_y739 CCCTATGCAGACCTTCCTTCCCCTATATCCAG(+AAAAAA)GATCCCTGCATCCTTTCCTGGATCTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240606171354_nvx4 AGCCCTGGAACAGATCCAGGAAAGGATGCAGG(+AAAAAA)GATCCTGGATATAGGGGAAGGAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10