Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171340_ne1i CCCGGGGGCCTTAGTTGTGAGGCATGGGTTGG(+AAAAAA)GATCCGCCAGCACCAGGGTGTCATCTAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606171338_vmm5 CTTCTGTTCTAGATGACACCCTGGTGCTGGCG(+AAAAAA)GATCCCAACCCATGCCTCACAACTAAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240606171336_d4ug TAATCCCTTAATTTGGAAGTTTTCTGTTCTGG(+AAAAAA)GATCCTCCCAAGAGGTTTTATAAGCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606171334_4x2h CTCTTAAACCAGCTTATAAAACCTCTTGGGAG(+AAAAAA)GATCCCAGAACAGAAAACTTCCAAATTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606171333_5yiv GTATGTAGTCTGTTTGTCTACACTGTGTATAG(+AAAAAA)GATCCACAGTGTAGAAAAGAGTGTTTATAGA NGG 10 8 17 8 24 0 100 5 10
    240606171332_8uet AATACTCTATAAACACTCTTTTCTACACTGTG(+AAAAAA)GATCCTATACACAGTGTAGACAAACAGACTA NGG 10 8 17 8 24 0 100 5 10
    240606171331_uhcy CTGGAAGCAAGCCCCCTCCTGGCATCTCTAGG(+AAAAAA)GTACCTGGGCCCACATTCCTGTGGCCCACAA NGG 10 8 17 8 24 0 100 5 10
    240606171328_kfu6 CCCATTTGTGGGCCACAGGAATGTGGGCCCAG(+AAAAAA)GTACCCTAGAGATGCCAGGAGGGGGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606171326_vf54 TTCCTGGAAGCCTGCCCCCAGCCCAGCCAGGG(+AAAAAA)GATCCAGTCAGCAGCGTGGAGGGATTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606171324_d3y0 AAGGCACTTGAATCCCTCCACGCTGCTGACTG(+AAAAAA)GATCCCCTGGCTGGGCTGGGGGCAGGCTTCC NGG 10 8 17 8 24 0 100 5 10