Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171253_h3f8 GATGGCGCTTTGGCAAACCCTCACTGGTCTGG(+AAAAAA)GTACCCGCTTTAGAGCAGCAGTCTCCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606171249_6mpt GTCCTTGGAGCTTTTCTTCTTTCCAGCCCGGG(+AAAAAA)GATCCCTTCCTCCTTCAGCTCTTGTCTGCAC NGG 10 8 17 8 24 0 100 5 10
    240606171247_xeap GTGGGGTGCAGACAAGAGCTGAAGGAGGAAGG(+AAAAAA)GATCCCCGGGCTGGAAAGAAGAAAAGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240606171246_zayc CTCCTTCTACAGTTTCTGAATTTTATAAAGAG(+AAAAAA)GTACCCTGTCAGGCAGAAAGTCCAGGACTAA NGG 10 8 17 8 24 0 100 5 10
    240606171244_3fxy GATGCTTAGTCCTGGACTTTCTGCCTGACAGG(+AAAAAA)GTACCTCTTTATAAAATTCAGAAACTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240606171243_r5nv GGCTAAGTTGAAACAGCCTGTATTCCCTCAAG(+AAAAAA)GATCCCTACTCTTGGCCCTCTGACCTAATGA NGG 10 8 17 8 24 0 100 5 10
    240606171241_507w ATACCTCATTAGGTCAGAGGGCCAAGAGTAGG(+AAAAAA)GATCCTTGAGGGAATACAGGCTGTTTCAACT NGG 10 8 17 8 24 0 100 5 10
    240606171240_0176 TGTCTCTTAAGTGCTAGGATTTAAGGTGTGTG(+AAAAAA)GTACCACATCTGGATGGCATTTGGCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606171239_q9d4 GAAGTTAAAAAGCCAAATGCCATCCAGATGTG(+AAAAAA)GTACCACACACCTTAAATCCTAGCACTTAAG NGG 10 8 17 8 24 0 100 5 10
    240606171237_n2q9 AAGGGCCAGAGCTGGGGGGCAAGAAGCTTGTG(+AAAAAA)GATCCTTCCCCCCATGCACTGTGAAGGACCC NGG 10 8 17 8 24 0 100 5 10