Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171217_1wyh CCCCTTTAAGCATGTGGTGTTGTCTTAGCATG(+AAAAAA)GTACCTGCTGGGTTTTTGTTGTTGTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606171216_ps4x ATAATATCAGTTTTATTAAATATTCGAGAACG(+AAAAAA)GATCCAGAGGTCGGATTTATACAGGAGGATA NGG 10 8 17 8 24 0 100 5 10
    240606171214_kbby CGTGTTATCCTCCTGTATAAATCCGACCTCTG(+AAAAAA)GATCCGTTCTCGAATATTTAATAAAACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606171212_5ytq AAGGCCAGGGGTTTAGAGACAGTCTTCCTCTG(+AAAAAA)GTACCCCATTCGAACCCACCTCTACAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606171209_pirx CATCCGCAGCTGTAGAGGTGGGTTCGAATGGG(+AAAAAA)GTACCAGAGGAAGACTGTCTCTAAACCCCTG NGG 10 8 17 8 24 0 100 5 10
    240606171208_fxpm TTTGTGCCTTCTGTGAGGGATAGGGGACAAGG(+AAAAAA)GATCCCAGGTGTCCCCATTTCCCGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606171205_wmtt AAGGTGAGGGGGCGGGAAATGGGGACACCTGG(+AAAAAA)GATCCCTTGTCCCCTATCCCTCACAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606171203_h7ei TGTGTCAGAGGTAGAGATGGCACCTCTGGAGG(+AAAAAA)GTACCAGAGCTAGGTGGATACATGGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606171202_m1ni TGCCCCTGGGTCCATGTATCCACCTAGCTCTG(+AAAAAA)GTACCCTCCAGAGGTGCCATCTCTACCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606171200_cntz CTCTTCAAAATCCAAAACCTGTGGCCTTACTG(+AAAAAA)GTACCAAATTCTCCTTGTAAACATACCTGGC NGG 10 8 17 8 24 0 100 5 10