Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171125_fx2i TAGAGCCCCTGGCTAGTCTCTTGGGACAGGCG(+AAAAAA)GTACCTGACTCTTCCACCCTCTGTCCCCTTA NGG 10 8 17 8 24 0 100 5 10
    240606171122_1qzr TTGACCAAGGGTAGGGAAGAGTGAAAGCCCAG(+AAAAAA)GATCCGGGCCCGGGATTCCCTGGCTTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240606171120_2pip CAGGGTGGCCAAGCCAGGGAATCCCGGGCCCG(+AAAAAA)GATCCTGGGCTTTCACTCTTCCCTACCCTTG NGG 10 8 17 8 24 0 100 5 10
    240606171118_qe9r GCCCTCCACTCCCTCTGCTAACATTGGGTTGG(+AAAAAA)GATCCTCTCTCGGTTGTAACCCATTTCTACC NGG 10 8 17 8 24 0 100 5 10
    240606171117_rtbt AATGAGGTAGAAATGGGTTACAACCGAGAGAG(+AAAAAA)GATCCCAACCCAATGTTAGCAGAGGGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606171115_de7p CAGAGATGTCTGTCAAACATGTATATGCAGCG(+AAAAAA)GATCCATCTGCTTGTTTGGTTGGGTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606171114_5tti CAACAAAAACAACCCAACCAAACAAGCAGATG(+AAAAAA)GATCCGCTGCATATACATGTTTGACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240606171112_xqz5 GTTGTTAGAGCAAAGACACCATTCCATTCCAG(+AAAAAA)GTACCAAGTGAAGCATACTTGACAAATAGGC NGG 10 8 17 8 24 0 100 5 10
    240606171111_1tdf AGACTGCCTATTTGTCAAGTATGCTTCACTTG(+AAAAAA)GTACCTGGAATGGAATGGTGTCTTTGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240606171110_xet6 TGCAGAGATGGTTTATGAGGCGGGTCCGAAGG(+AAAAAA)GTACCCTTCACCTTGCCTGGAGATATACAAA NGG 10 8 17 8 24 0 100 5 10