Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171053_esva CCCAGGCTGGCCTCCAATCCCTGTGCTCAAGG(+AAAAAA)GATCCTCTCTCTCCAGACTCGGGTGCTGCGG NGG 10 8 17 8 24 0 100 5 10
    240606171052_2tcu TGTGACTGAGCTAGCCAGCCAGTAAGTTCAAG(+AAAAAA)GATCCTCCTGGCCTCGCGCCCCAACACTGTG NGG 10 8 17 8 24 0 100 5 10
    240606171050_4xjf GGGACCACAGTGTTGGGGCGCGAGGCCAGGAG(+AAAAAA)GATCCTTGAACTTACTGGCTGGCTAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606171048_cupq ATCTTCATTCCTGAGCCACCTTGCCTCCTCTG(+AAAAAA)GATCCCTTTTCTAAGAAAACACACTTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606171047_666t AGTGCAGACCAAGTGTGTTTTCTTAGAAAAGG(+AAAAAA)GATCCAGAGGAGGCAAGGTGGCTCAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240606171046_izj6 AAAATATATCGGCAGTCACTTCTTTTATAAAG(+AAAAAA)GTACCATGGGCTACATTACACAATAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606171043_nqve TACATTGAGACTATTGTGTAATGTAGCCCATG(+AAAAAA)GTACCTTTATAAAAGAAGTGACTGCCGATAT NGG 10 8 17 8 24 0 100 5 10
    240606171042_tpmh GTTTGATACCCAGGCCTTGCTCTGCTTAGTAG(+AAAAAA)GTACCATGGGCCGTTTTAAGAGCTACACATG NGG 10 8 17 8 24 0 100 5 10
    240606171039_3r0s TCTGCCATGTGTAGCTCTTAAAACGGCCCATG(+AAAAAA)GTACCTACTAAGCAGAGCAAGGCCTGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240606171038_rfzw CACTGAGTCAGGGCTCCGGCTTCTGGCTGTGG(+AAAAAA)GTACCCACCGCCCAGCTGCAAGAGGGAGTCA NGG 10 8 17 8 24 0 100 5 10