Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170947_kpmt CCTGGAGACAGCCACTGTAACTCCTAGAAAGG(+AAAAAA)GATCCAAGACAGCTTTTCCGCCGGGGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606170945_lu5q GCATGGTGTGCTTTCTGACTGGGGTAGTGTGG(+AAAAAA)GATCCCTCTCTGAGGACCCCGATGTTGGTAC NGG 10 8 17 8 24 0 100 5 10
    240606170944_3uvh CCCTAGTACCAACATCGGGGTCCTCAGAGAGG(+AAAAAA)GATCCCACACTACCCCAGTCAGAAAGCACAC NGG 10 8 17 8 24 0 100 5 10
    240606170943_baxv TGGAAACTTGGAAGAAGAGTGGTACTGTGATG(+AAAAAA)GTACCTGATGGAGTGGTCCAAATTCCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240606170941_hlbg GAAACAACTGGGAATTTGGACCACTCCATCAG(+AAAAAA)GTACCATCACAGTACCACTCTTCTTCCAAGT NGG 10 8 17 8 24 0 100 5 10
    240606170940_hvn2 TCTGGAGAAACTCACGACAGAGGTGGTGCTTG(+AAAAAA)GTACCTAGTTCCCAGGGCCAGGTAAACTTGT NGG 10 8 17 8 24 0 100 5 10
    240606170939_xgd7 GTGACACAAGTTTACCTGGCCCTGGGAACTAG(+AAAAAA)GTACCAAGCACCACCTCTGTCGTGAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606170938_aq6o CCACTGCTGCGACACGTGGAGGCCCCACAGTG(+AAAAAA)GTACCCCAGAGTGGGAAGGTTGGATGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606170936_jnxn TCCCATGAGCCATCCAACCTTCCCACTCTGGG(+AAAAAA)GTACCACTGTGGGGCCTCCACGTGTCGCAGC NGG 10 8 17 8 24 0 100 5 10
    240606170935_hefg GAGGTCCATTCTCACTGGTTGGGGTCCATGTG(+AAAAAA)GTACCAGTTCCCAAGTATTTACTCTACACAG NGG 10 8 17 8 24 0 100 5 10