Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170846_4u1v AGACTTGACCAGCAAGAACGACGCTGCAACAG(+AAAAAA)GATCCTTCTGCACACGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606170845_psfh GGCCTTTGCTGAGGCATTCCTTTCCCTCTGAG(+AAAAAA)GTACCAGTCACAGGACGGGATAATATAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606170843_n141 ACTCTATTCTATATTATCCCGTCCTGTGACTG(+AAAAAA)GTACCTCAGAGGGAAAGGAATGCCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606170842_zxnq GAGCAAGAGTTTATAGAGCTGGGGGATGTACG(+AAAAAA)GTACCTACCTCTGTATGTGACAGAGGACTCA NGG 10 8 17 8 24 0 100 5 10
    240606170841_wela GGATATGAGTCCTCTGTCACATACAGAGGTAG(+AAAAAA)GTACCGTACATCCCCCAGCTCTATAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240606170839_wrep CTCCTGGAAGAGAAAGGTCATAGAACACATAG(+AAAAAA)GTACCCCACCCCTCTGGAAGGCAGAGGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606170837_9li2 TGTAATTACCCTCTGCCTTCCAGAGGGGTGGG(+AAAAAA)GTACCTATGTGTTCTATGACCTTTCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606170836_80cj CATCCAATTCATTTGAATATCTTATATTGTGG(+AAAAAA)GTACCTTTTTTAAATAGAAAACTTAAGATGA NGG 10 8 17 8 24 0 100 5 10
    240606170835_1d5z AAGAATCATCTTAAGTTTTCTATTTAAAAAAG(+AAAAAA)GTACCCACAATATAAGATATTCAAATGAATT NGG 10 8 17 8 24 0 100 5 10
    240606170833_hh77 TTCATAGGTGAAATCTTGCCTCCCAGTGTAAG(+AAAAAA)GTACCTGGTTTCTTTTTGTGATACTTCTCTT NGG 10 8 17 8 24 0 100 5 10