Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170900_jco9 GAAGTCTCTCCACACTGATCGGCTGTGAACAG(+AAAAAA)GATCCTGGTTGTCATGGCACCTCTCAGGATG NGG 10 8 17 8 24 0 100 5 10
    240606170859_lyzg TGAATATAAGGGATCTTACTGTCATTTGTTAG(+AAAAAA)GTACCACTGAGTAATTCCATAGTTTCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606170858_qm2x AATTACCACAGAAACTATGGAATTACTCAGTG(+AAAAAA)GTACCTAACAAATGACAGTAAGATCCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240606170855_4fva TGCCTGCAGTTTTAACTTTATAGCTCTCCCAG(+AAAAAA)GTACCAGCTTTGGGGCCAGCACACGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240606170854_9ki2 AATGAGAGTGCCGTGTGCTGGCCCCAAAGCTG(+AAAAAA)GTACCTGGGAGAGCTATAAAGTTAAAACTGC NGG 10 8 17 8 24 0 100 5 10
    240606170853_qjk9 CCTCCAGGTGAGACCTGTGATTTCAAGCTTGG(+AAAAAA)GTACCACTCTAAGCTTTCTCCGGTCAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606170851_5ipr GAGGATGGGCTGACCGGAGAAAGCTTAGAGTG(+AAAAAA)GTACCCAAGCTTGAAATCACAGGTCTCACCT NGG 10 8 17 8 24 0 100 5 10
    240606170850_mkph CCAAAATGGAATTGCTGTTTCCCAAACTTTGG(+AAAAAA)GATCCTTGAAGGGAGAGCTGAACATTAATAT NGG 10 8 17 8 24 0 100 5 10
    240606170849_t3qi TGCTCATATTAATGTTCAGCTCTCCCTTCAAG(+AAAAAA)GATCCCAAAGTTTGGGAAACAGCAATTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606170847_tbme AATCAAGCTCTCCCAATAAACGTGTGCAGAAG(+AAAAAA)GATCCTGTTGCAGCGTCGTTCTTGCTGGTCA NGG 10 8 17 8 24 0 100 5 10