Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170750_0ezk TCCATCGTGGCCCCAAACTAGAGATAATGCAG(+AAAAAA)GTACCCACTGATTGTTAAGTAAATAAGTATG NGG 10 8 17 8 24 0 100 5 10
    240606170749_dil8 CACAACTCATTGAAGATAGAGAAGTTGAGCTG(+AAAAAA)GTACCTATGTAGAGAGCCTCCACCCCTACAT NGG 10 8 17 8 24 0 100 5 10
    240606170748_ppy2 CTAGAATGTAGGGGTGGAGGCTCTCTACATAG(+AAAAAA)GTACCAGCTCAACTTCTCTATCTTCAATGAG NGG 10 8 17 8 24 0 100 5 10
    240606170746_qkgu TCTTGTACAAGGAAGTGGAGTGACAGTGGGTG(+AAAAAA)GTACCACTTCCCAGTGAAAGGAAGGTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240606170745_cvri TCTCCTCCTAACCTTCCTTTCACTGGGAAGTG(+AAAAAA)GTACCACCCACTGTCACTCCACTTCCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606170743_dab7 ACCTACAGAGCACAGGGCACCTCAGCTATTGG(+AAAAAA)GTACCGGAACACAAACACCAGTAACTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240606170741_bswi CCATGTGAGGAGTTACTGGTGTTTGTGTTCCG(+AAAAAA)GTACCCAATAGCTGAGGTGCCCTGTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240606170740_iwgn GAAAGAAAACCACATGTCTTGCCTCATCTGTG(+AAAAAA)GATCCTGGCCTATAATGTGCACATGTAGACT NGG 10 8 17 8 24 0 100 5 10
    240606170738_frdf CCCTGAGTCTACATGTGCACATTATAGGCCAG(+AAAAAA)GATCCACAGATGAGGCAAGACATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240606170737_fr8u TGCAGGCACCAGGCACGAATGTGGAGAGCATG(+AAAAAA)GATCCGTACAGGCACTCCCATATGCACAAAA NGG 10 8 17 8 24 0 100 5 10