Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170647_4mxz GCTCTCACAATGTGGCTCACAACCTTCCCCTG(+AAAAAA)GATCCTTTTTACACATAAGATGTACAGATGC NGG 10 8 17 8 24 0 100 5 10
    240606170646_1rtn CTGGTACCCGTGAAGGTCAGAAGAGGGTGAAG(+AAAAAA)GATCCTCTGGAACTGGAGTTACCGATGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606170644_sh9j GTTTATAACCATCGGTAACTCCAGTTCCAGAG(+AAAAAA)GATCCTTCACCCTCTTCTGACCTTCACGGGT NGG 10 8 17 8 24 0 100 5 10
    240606170642_u7d9 ACACACAGCAAACCCCACGGCTTCCCAGACGG(+AAAAAA)GATCCTTCTGCTCCCTTCTACGAGGGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606170641_bfdj AGGTAGTCTGCCCTCGTAGAAGGGAGCAGAAG(+AAAAAA)GATCCCGTCTGGGAAGCCGTGGGGTTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606170640_etdv ATGCTTTCTAAGGATGCTCGATAAACTTATTG(+AAAAAA)GATCCCCAGAGAGCACGTTGGCAGAATCATG NGG 10 8 17 8 24 0 100 5 10
    240606170637_2ita TGAAGCATGATTCTGCCAACGTGCTCTCTGGG(+AAAAAA)GATCCAATAAGTTTATCGAGCATCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606170636_yahv ACCTGGAATTTGCCTATGCTCAGTCTTGGATG(+AAAAAA)GATCCGGAATAGTTTCATGGAACAGTGATAA NGG 10 8 17 8 24 0 100 5 10
    240606170634_hurg GGCAATTATCACTGTTCCATGAAACTATTCCG(+AAAAAA)GATCCATCCAAGACTGAGCATAGGCAAATTC NGG 10 8 17 8 24 0 100 5 10
    240606170632_718e CAGATCTTCCCACCTCAGGCTCCAAGTGCTGG(+AAAAAA)GATCCCAACCCTAACCCTAAGGGACAGATTT NGG 10 8 17 8 24 0 100 5 10