Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170613_dkqc ACTCCGTCCGTCCGTCACAGTGAGGGCCAGGG(+AAAAAA)GATCCTTGGAGAGGAACAGAGAGCTTGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606170612_kwf2 CGTGCTCTTAGGTCGTTTTATTTTACAATCTG(+AAAAAA)GATCCGAGGTGGCAGATTTAAGACAGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606170610_ydd7 TCTACAGAACCTGTCTTAAATCTGCCACCTCG(+AAAAAA)GATCCAGATTGTAAAATAAAACGACCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240606170608_vla7 ATGCAACAGCCTGCAAAGGCCATAGGGTGTTG(+AAAAAA)GATCCCTGGAACTGGAGTTACAGGAGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606170606_5h42 TTTCCTCAACCTCCTGTAACTCCAGTTCCAGG(+AAAAAA)GATCCAACACCCTATGGCCTTTGCAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606170604_4n9j ACAAAAACCCTCTATTAAACACAACAGAAAAG(+AAAAAA)GATCCACAGTGCATCCAGACAGTGGAGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606170603_seqh GGCTGCCAACTCCACTGTCTGGATGCACTGTG(+AAAAAA)GATCCTTTTCTGTTGTGTTTAATAGAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240606170600_ctuu AGCTAACACCATCTGCAGCTCCTGTCCCAGGG(+AAAAAA)GATCCGCTGTTCTCATGGCCTCTGTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240606170559_lt9s GTCCAGTGCCCACAGAGGCCATGAGAACAGCG(+AAAAAA)GATCCCCTGGGACAGGAGCTGCAGATGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606170558_o85w TCAGAAACGAAGTTCACACTGAAGCTCGAAAG(+AAAAAA)GTACCAGACAGTGCAGGCTTCCCATCCCATC NGG 10 8 17 8 24 0 100 5 10