Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170529_ilov AGTCAAGCAACAGCCAGAACAAGCATTGTTAG(+AAAAAA)GATCCTCCTCTTCCGTATGGGTAATTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606170528_lm5j GTTTCAGCAGTCCTGGAAAATGTGCTTTCTTG(+AAAAAA)GATCCAAATTACATTCTCTTCCTAAACCAAA NGG 10 8 17 8 24 0 100 5 10
    240606170526_sh1v ACAGTTTTGGTTTAGGAAGAGAATGTAATTTG(+AAAAAA)GATCCAAGAAAGCACATTTTCCAGGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240606170525_gfh3 GGGTCTCTAGATAACGCTACCCTGAATCAGAG(+AAAAAA)GATCCCTAAACCCAATGGCATTAACTGCACA NGG 10 8 17 8 24 0 100 5 10
    240606170523_pu5j CTTCTTGTGCAGTTAATGCCATTGGGTTTAGG(+AAAAAA)GATCCTCTGATTCAGGGTAGCGTTATCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606170522_4kti GATACCCACCATCTTCCTGCATCAGGATGAGG(+AAAAAA)GATCCGTGTCATTCACACACACACCCCGACC NGG 10 8 17 8 24 0 100 5 10
    240606170521_vb95 GTGGGGGTCGGGGTGTGTGTGTGAATGACACG(+AAAAAA)GATCCCTCATCCTGATGCAGGAAGATGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606170519_7wt1 CTTCCCGAGCTCCTTGTGAGTTTGGGGGCTAG(+AAAAAA)GATCCCTTCCTGTGATCAAAGGTGGGACGAT NGG 10 8 17 8 24 0 100 5 10
    240606170518_nl45 AAGGGATCGTCCCACCTTTGATCACAGGAAGG(+AAAAAA)GATCCTAGCCCCCAAACTCACAAGGAGCTCG NGG 10 8 17 8 24 0 100 5 10
    240606170516_sfxa GGGCCAGCTGACGAGTTCCCCATCATCCTGAG(+AAAAAA)GATCCAACCCCACCTCAAGTTTCTCCTGGAG NGG 10 8 17 8 24 0 100 5 10