Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170514_j86g GGAGGCTCCAGGAGAAACTTGAGGTGGGGTTG(+AAAAAA)GATCCTCAGGATGATGGGGAACTCGTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606170513_1xwd GGGCCTGGGCCAAAACCAGCAAGGTATAGTGG(+AAAAAA)GATCCAGGAGGGACTGAGGGAGCCAGGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606170511_uvpj CCTTAGGCCCCTGGCTCCCTCAGTCCCTCCTG(+AAAAAA)GATCCCACTATACCTTGCTGGTTTTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606170508_7j3d CACGTGTCCTAATGATGAAGTCAGAGCCCCGG(+AAAAAA)GATCCCAGCACACACCGTGACACAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606170506_jo8v CAGTGGCTGGGCTGTGTCACGGTGTGTGCTGG(+AAAAAA)GATCCCGGGGCTCTGACTTCATCATTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606170505_h4hf CCGTCAATGCCGCCTCAGGATGCAGGTGTGTG(+AAAAAA)GTACCATGGCCTTGGGCAGCCCGTTGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606170503_ip3y CATCTCAGGCCAACGGGCTGCCCAAGGCCATG(+AAAAAA)GTACCACACACCTGCATCCTGAGGCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606170502_fpkz AAGCAAATGTAAGGGTATAGGAGCAGCAGCAG(+AAAAAA)GATCCTGAGTCACAAGGGCACAATGCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606170500_1tpc TCTCCAGCTGGCATTGTGCCCTTGTGACTCAG(+AAAAAA)GATCCTGCTGCTGCTCCTATACCCTTACATT NGG 10 8 17 8 24 0 100 5 10
    240606170458_8x2d GGCTTCAGTTCTCCCAAAGAGCCAGCCCCTTG(+AAAAAA)GATCCAGTGCGCCCAGCCTTTCCCATGTCTA NGG 10 8 17 8 24 0 100 5 10