Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170423_f6y0 CTCTTAAAGCAAGCTCTGGAACTCCCCCACAG(+AAAAAA)GATCCCCTCTAGCCCTGGCCTGCAGGAGGAC NGG 10 8 17 8 24 0 100 5 10
    240606170422_ls2y GAGTGGCATTTGTCTAAGATTCACCAGAACAG(+AAAAAA)GATCCTGTCCTCCTAGGGGACCTTCCAAACA NGG 10 8 17 8 24 0 100 5 10
    240606170421_9huy TCCTATGTTTGGAAGGTCCCCTAGGAGGACAG(+AAAAAA)GATCCTGTTCTGGTGAATCTTAGACAAATGC NGG 10 8 17 8 24 0 100 5 10
    240606170420_wwjg CCAGCATCCAAGGTCAACGCAATGGTGGTCTG(+AAAAAA)GTACCCAATGGAGACAAATCCCTTTACTTCT NGG 10 8 17 8 24 0 100 5 10
    240606170418_q60v CAAAGAGAAGTAAAGGGATTTGTCTCCATTGG(+AAAAAA)GTACCAGACCACCATTGCGTTGACCTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606170416_ji1p CATGTCTCTTAAGTTATTGTGACCTACACTGG(+AAAAAA)GTACCGGAGGGGATGGGGGATGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606170414_8l69 ACAGCAGCTGAAGCCATCCCCCATCCCCTCCG(+AAAAAA)GTACCCAGTGTAGGTCACAATAACTTAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606170412_zmgi TGTGGCACTCAAGCATACTGATCCCCGCTCAG(+AAAAAA)GTACCACCATTCCCTATGTAGAGGAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606170411_j6lc GGGTGCTTCCCTCCTCTACATAGGGAATGGTG(+AAAAAA)GTACCTGAGCGGGGATCAGTATGCTTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606170410_2jju ATAGAACACTCAAAAGGGACGTGAATGTGCAG(+AAAAAA)GTACCATGGGTCTGGGGTGACCTGCTTCACA NGG 10 8 17 8 24 0 100 5 10