Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170457_p5c1 CGCAGTAGACATGGGAAAGGCTGGGCGCACTG(+AAAAAA)GATCCAAGGGGCTGGCTCTTTGGGAGAACTG NGG 10 8 17 8 24 0 100 5 10
    240606170455_4plh TGGTGAAGTCCTAAGCATGACTTTAAAACCTG(+AAAAAA)GATCCAATTTCAAGGGGCTCATGATAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606170454_e18w ATATCCCACTTATCATGAGCCCCTTGAAATTG(+AAAAAA)GATCCAGGTTTTAAAGTCATGCTTAGGACTT NGG 10 8 17 8 24 0 100 5 10
    240606170452_41eu AATCTCTGCATGAGTGAAGGGAGGACACATGG(+AAAAAA)GTACCGGAATGGGTCTCACAGAAATGGACTT NGG 10 8 17 8 24 0 100 5 10
    240606170451_na6q AAAGAAAGTCCATTTCTGTGAGACCCATTCCG(+AAAAAA)GTACCCATGTGTCCTCCCTTCACTCATGCAG NGG 10 8 17 8 24 0 100 5 10
    240606170449_1xvc AGGCTCCACACACCAGGTATTGCTGATTCCAG(+AAAAAA)GTACCACCCTTGACATGTGTGTCTGAGTCCC NGG 10 8 17 8 24 0 100 5 10
    240606170447_8upv AGGCTGGGACTCAGACACACATGTCAAGGGTG(+AAAAAA)GTACCTGGAATCAGCAATACCTGGTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606170446_sdvl CAGCTTTGTCAACAGTTCTGTATCCATACCTG(+AAAAAA)GTACCCTCGGCCCCGCTTCCGAGTCGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606170443_s7n5 AAGTGAAAAGCGACTCGGAAGCGGGGCCGAGG(+AAAAAA)GTACCAGGTATGGATACAGAACTGTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240606170442_5pjh AGAAGACACCCAGGGTGACGTCTCATCCTCAG(+AAAAAA)GATCCCCCACGTCTGCTGCCATCACAGCCGC NGG 10 8 17 8 24 0 100 5 10