Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170544_2gn8 AATTTCCATGCTTCCTAAACTAGCTGATATCG(+AAAAAA)GATCCTTCTGAGGAACTCATTTAAAGCAGAA NGG 10 8 17 8 24 0 100 5 10
    240606170541_66if GTCCACAGCTCTCTGTAACTCCAGTGCCAAGG(+AAAAAA)GATCCAACACACTCTTCTAACCTCTGTGGAC NGG 10 8 17 8 24 0 100 5 10
    240606170540_vwjw CTGGTGTCCACAGAGGTTAGAAGAGTGTGTTG(+AAAAAA)GATCCCTTGGCACTGGAGTTACAGAGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606170539_be4w CACAAGTTAAGGAAAGATATTAGCCAAAGAAG(+AAAAAA)GTACCAGCCTATGAAAAACAAATTAAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240606170538_vi1p CTGGTACTTTTTAATTTGTTTTTCATAGGCTG(+AAAAAA)GTACCTTCTTTGGCTAATATCTTTCCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606170536_pu5o GCACACAGGTATCTCAACAGAGCTGGCACAGG(+AAAAAA)GTACCTTTTTTTAAAGGGGCAGCTAGAATCC NGG 10 8 17 8 24 0 100 5 10
    240606170535_af2m ACTAAGGATTCTAGCTGCCCCTTTAAAAAAAG(+AAAAAA)GTACCCTGTGCCAGCTCTGTTGAGATACCTG NGG 10 8 17 8 24 0 100 5 10
    240606170533_sder ACATTACTGTTGTACCCAGTGACTTCACTTGG(+AAAAAA)GATCCCTGTCTCCTTAAACCACATATAATTA NGG 10 8 17 8 24 0 100 5 10
    240606170532_splz ACTAATAATTATATGTGGTTTAAGGAGACAGG(+AAAAAA)GATCCCAAGTGAAGTCACTGGGTACAACAGT NGG 10 8 17 8 24 0 100 5 10
    240606170530_3bg6 TTAAAAAGAGAATTACCCATACGGAAGAGGAG(+AAAAAA)GATCCTAACAATGCTTGTTCTGGCTGTTGCT NGG 10 8 17 8 24 0 100 5 10