Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170631_3e8k GACATAAATCTGTCCCTTAGGGTTAGGGTTGG(+AAAAAA)GATCCCAGCACTTGGAGCCTGAGGTGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606170629_5cof GTCTATTTGATGAGAGAATAACTGGGCACCAG(+AAAAAA)GATCCTATTTTGGAAGCTTCACTTATGTACC NGG 10 8 17 8 24 0 100 5 10
    240606170627_3bzd CATATGGTACATAAGTGAAGCTTCCAAAATAG(+AAAAAA)GATCCTGGTGCCCAGTTATTCTCTCATCAAA NGG 10 8 17 8 24 0 100 5 10
    240606170626_8kql GCAAGTGCTCATGACAGTTGCGGGGAAGACCG(+AAAAAA)GATCCGGAGGTGTTACAAGCTTTGATAAATG NGG 10 8 17 8 24 0 100 5 10
    240606170623_wzqp CTGGCCATTTATCAAAGCTTGTAACACCTCCG(+AAAAAA)GATCCGGTCTTCCCCGCAACTGTCATGAGCA NGG 10 8 17 8 24 0 100 5 10
    240606170622_vl7b GGTTTAGGGAGATCTGGTTGAGTTATCAAGTG(+AAAAAA)GATCCTAAAGGAAATTCTTGAGCCTAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606170621_14zm CATTTTCCTTTAGGCTCAAGAATTTCCTTTAG(+AAAAAA)GATCCACTTGATAACTCAACCAGATCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606170619_za9n AAGGCTCTAAAGTGAGTAGGTCATTCCCTAGG(+AAAAAA)GATCCTACAGATCACCCCCTGTTAAATCTAC NGG 10 8 17 8 24 0 100 5 10
    240606170617_k0gd TATAGGTAGATTTAACAGGGGGTGATCTGTAG(+AAAAAA)GATCCCTAGGGAATGACCTACTCACTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240606170615_61h3 CCAGAGTGCCAAGCTCTCTGTTCCTCTCCAAG(+AAAAAA)GATCCCCTGGCCCTCACTGTGACGGACGGAC NGG 10 8 17 8 24 0 100 5 10