Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170736_u4ci TTATTTTTTGTGCATATGGGAGTGCCTGTACG(+AAAAAA)GATCCATGCTCTCCACATTCGTGCCTGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606170735_v34g GATTCCTGATTCCTTCTCAACATGTGTGTAAG(+AAAAAA)GATCCTCGACCTGCAAAGTCCATCTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240606170733_31an TTCATGGAAAGAGATGGACTTTGCAGGTCGAG(+AAAAAA)GATCCTTACACACATGTTGAGAAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240606170732_m218 GCCAACACCAAACGAATCAGTAGCAGCTGCTG(+AAAAAA)GATCCAGCAGAAACCACAAGGCTCCACCGAA NGG 10 8 17 8 24 0 100 5 10
    240606170731_j5l2 GCAGATTCGGTGGAGCCTTGTGGTTTCTGCTG(+AAAAAA)GATCCAGCAGCTGCTACTGATTCGTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240606170730_uoyo AGATCCTGACTTTGGCTCCAGCTATCAGTTGG(+AAAAAA)GATCCTCAACGGACCACAATGAAGGTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606170728_epgm AAAGTGGAGGACCTTCATTGTGGTCCGTTGAG(+AAAAAA)GATCCCAACTGATAGCTGGAGCCAAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606170727_fajb TGAAGTGTTGTGGAAGCCAAAAGAGAGTTTTG(+AAAAAA)GATCCCCTGGAGTTCCTGGGGAGGAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606170725_lhj0 GAGTTTGGCTCTCCTCCCCAGGAACTCCAGGG(+AAAAAA)GATCCAAAACTCTCTTTTGGCTTCCACAACA NGG 10 8 17 8 24 0 100 5 10
    240606170724_e73n CATGTGCCTTCTGAGTCTAGAAGAGGGTATTG(+AAAAAA)GATCCCATGGAGCTGAAATTATAGGCAGTCG NGG 10 8 17 8 24 0 100 5 10