Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170706_zboe TTACCCTGCCTTAAGCTCCTGGCCGACCCTTG(+AAAAAA)GATCCACACATGAAACACATACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606170703_hyrj ACCCGCAAGCTCCAGAAACGGCCCCACCTGGG(+AAAAAA)GATCCATCCCATTTACAGTTACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606170701_63yj AGTGTCTGGGTTTGGTAACTGTAAATGGGATG(+AAAAAA)GATCCCCAGGTGGGGCCGTTTCTGGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606170659_15bb AGACCACCTGCTAAAAGGCTCAGAGACAGAGG(+AAAAAA)GATCCTGGCTCTTTTATACAACTAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606170657_hmpe GTGTCTTTTTCTTAGTTGTATAAAAGAGCCAG(+AAAAAA)GATCCCTCTGTCTCTGAGCCTTTTAGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606170656_atau TTCTACACAGAATATTTTCCCCATATTTATTG(+AAAAAA)GATCCAACCTGCTTACGGGATCTGTTAGTGC NGG 10 8 17 8 24 0 100 5 10
    240606170654_wt0u TTACAGCACTAACAGATCCCGTAAGCAGGTTG(+AAAAAA)GATCCAATAAATATGGGGAAAATATTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606170653_acln CTGTGGAGCATTTTCTTGATTGCTAATTGGAG(+AAAAAA)GATCCAACCCACCGTGGGTGTTACTATCCCT NGG 10 8 17 8 24 0 100 5 10
    240606170651_ga65 TGCTTAGGGATAGTAACACCCACGGTGGGTTG(+AAAAAA)GATCCTCCAATTAGCAATCAAGAAAATGCTC NGG 10 8 17 8 24 0 100 5 10
    240606170650_7m5u AGACAGCATCTGTACATCTTATGTGTAAAAAG(+AAAAAA)GATCCAGGGGAAGGTTGTGAGCCACATTGTG NGG 10 8 17 8 24 0 100 5 10