Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170721_7pf0 AGTCACGACTGCCTATAATTTCAGCTCCATGG(+AAAAAA)GATCCAATACCCTCTTCTAGACTCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606170720_u1sq TCCAAAGAAGCTAGAAGTTTCCATTTCACAGG(+AAAAAA)GATCCTGCCCAGCTCCTCGGGGTGGCTACCT NGG 10 8 17 8 24 0 100 5 10
    240606170718_1tst TTGTGAGGTAGCCACCCCGAGGAGCTGGGCAG(+AAAAAA)GATCCCTGTGAAATGGAAACTTCTAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606170717_2s53 CCGTCCATCAGTTCCATCTCTGTCTTCTATAG(+AAAAAA)GATCCCTTCTTAACAGTTTTTAGCATATTTT NGG 10 8 17 8 24 0 100 5 10
    240606170715_pa2j CACTAAAAATATGCTAAAAACTGTTAAGAAGG(+AAAAAA)GATCCTATAGAAGACAGAGATGGAACTGATG NGG 10 8 17 8 24 0 100 5 10
    240606170714_yy5p TATTTTAATATGTTATACATCAGTGTATGTAG(+AAAAAA)GATCCAGAGCTTTCCTTCTGAACCTCACTTC NGG 10 8 17 8 24 0 100 5 10
    240606170713_mw3t TTTCTGAAGTGAGGTTCAGAAGGAAAGCTCTG(+AAAAAA)GATCCTACATACACTGATGTATAACATATTA NGG 10 8 17 8 24 0 100 5 10
    240606170712_x26z AGATCAGGCCCAGTAGTCTCTAAGAGAGTGAG(+AAAAAA)GATCCTGAGGGAGACTATCCAGCTGCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606170710_qq9g GATCTGCCCTGCAGCTGGATAGTCTCCCTCAG(+AAAAAA)GATCCTCACTCTCTTAGAGACTACTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606170709_bgc3 GTGTGTGTGTGTGTGTATGTGTTTCATGTGTG(+AAAAAA)GATCCAAGGGTCGGCCAGGAGCTTAAGGCAG NGG 10 8 17 8 24 0 100 5 10