Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171036_9770 ATTGGTGACTCCCTCTTGCAGCTGGGCGGTGG(+AAAAAA)GTACCCACAGCCAGAAGCCGGAGCCCTGACT NGG 10 8 17 8 24 0 100 5 10
    240606171035_2qtm TTTAAGTCCCCAGGGTAGCTGGGGGCAGTTAG(+AAAAAA)GTACCCCTTGCTCCCCCAGCCCTCCCCACCT NGG 10 8 17 8 24 0 100 5 10
    240606171033_1ym7 GGCCTAGGTGGGGAGGGCTGGGGGAGCAAGGG(+AAAAAA)GTACCTAACTGCCCCCAGCTACCCTGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606171031_klau TTGGGACCCAATTCCCCTTTCTCCAAGACAGG(+AAAAAA)GATCCAGCGGCCCAGGATGCCCACTGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606171030_qhij AGCAGGACCCCAGTGGGCATCCTGGGCCGCTG(+AAAAAA)GATCCCTGTCTTGGAGAAAGGGGAATTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606171028_8fma TGCTCCCATCCCATCCCCGTGTGCGGCATGGG(+AAAAAA)GATCCAATGCTGCCTCTCCAGCTGAGAGGAT NGG 10 8 17 8 24 0 100 5 10
    240606171026_4wur CTAGCATCCTCTCAGCTGGAGAGGCAGCATTG(+AAAAAA)GATCCCCATGCCGCACACGGGGATGGGATGG NGG 10 8 17 8 24 0 100 5 10
    240606171022_udj8 TTCCATACCCTTTCTTCTGTGTTAGCCTGCAG(+AAAAAA)GATCCCTTAAGTACCCTTATTCCAATAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606171020_6n2n ACCCTGGCCTATTGGAATAAGGGTACTTAAGG(+AAAAAA)GATCCTGCAGGCTAACACAGAAGAAAGGGTA NGG 10 8 17 8 24 0 100 5 10
    240606171018_ia42 CCGTGGAGCTTTAACACGGAGAATCGAGTTTG(+AAAAAA)GATCCCAGAGACATCAGGCAGCCTTGAGACC NGG 10 8 17 8 24 0 100 5 10