Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606171236_fb54 AACGAGGGTCCTTCACAGTGCATGGGGGGAAG(+AAAAAA)GATCCACAAGCTTCTTGCCCCCCAGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606171234_yfih CATTCTTTTCACCAGCACCCATCGCCCAAGGG(+AAAAAA)GTACCAAGGGGGGCAAGGGGTGTCCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240606171231_erw0 GGGCTTGGACTGGACACCCCTTGCCCCCCTTG(+AAAAAA)GTACCCCTTGGGCGATGGGTGCTGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240606171229_6dgw CCATCCTCCCGCAGGAGGAGGGGAACAGGCGG(+AAAAAA)GATCCTGGGTCCCTCGTAAGCACTTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606171227_x19a CGGTAAAACCAAAGTGCTTACGAGGGACCCAG(+AAAAAA)GATCCCGCCTGTTCCCCTCCTCCTGCGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606171225_v64g GGTTTCCTTTTCCCCTACTTGACCTTCTATTG(+AAAAAA)GTACCCATGAAAAAGCACAAGGTCCTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606171224_73fv ACAGGGAGCTAGGACCTTGTGCTTTTTCATGG(+AAAAAA)GTACCAATAGAAGGTCAAGTAGGGGAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606171221_0z2v CCTCTTCCCCAGTCAGTGGACAATCTTTCCAG(+AAAAAA)GATCCCTGCTCATCCTTACAGATCATCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606171220_zebb AGTCTGCCAGATGATCTGTAAGGATGAGCAGG(+AAAAAA)GATCCTGGAAAGATTGTCCACTGACTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606171218_wfhi AAAACAAAACAACAACAACAAAAACCCAGCAG(+AAAAAA)GTACCATGCTAAGACAACACCACATGCTTAA NGG 10 8 17 8 24 0 100 5 10