Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165241_ax7e CCTGCTGTGACTAGATGTCTCAGAGCAGGATG(+AAAAAA)GTACCCAACGGGGCCTCCCCTGAGGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240606165239_8mzv CCTCCCCTTCTCCTCAGGGGAGGCCCCGTTGG(+AAAAAA)GTACCATCCTGCTCTGAGACATCTAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240606165237_sgv4 GCTGATTACATTCCTCAGAAAGACCACAGTGG(+AAAAAA)GTACCGACCATTGGCCACCTGTGGATAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606165236_73w2 GGCTTCTCTTATCCACAGGTGGCCAATGGTCG(+AAAAAA)GTACCCACTGTGGTCTTTCTGAGGAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240606165235_nwjs AGAGCAGTGATTCCGAGGAATGCCTCCAGCAG(+AAAAAA)GTACCGAGGTTCTTGTCTGTCTTGCTGTTTG NGG 10 8 17 8 24 0 100 5 10
    240606165233_9ulc TTTGACAAACAGCAAGACAGACAAGAACCTCG(+AAAAAA)GTACCTGCTGGAGGCATTCCTCGGAATCACT NGG 10 8 17 8 24 0 100 5 10
    240606165231_xnra CTTCTGATTGATGGTACCATCCCCCAAGAAGG(+AAAAAA)GATCCTCTCAGGAAGAAATGCAGGCTTTCTA NGG 10 8 17 8 24 0 100 5 10
    240606165230_lvyi GAGTTTAGAAAGCCTGCATTTCTTCCTGAGAG(+AAAAAA)GATCCCTTCTTGGGGGATGGTACCATCAATC NGG 10 8 17 8 24 0 100 5 10
    240606165228_vwvy CCTCTGTAGACCAGGCTGGCCATAAACTCAGG(+AAAAAA)GATCCACCTGCCTCTACTTTCGGAGTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606165227_4luf TAATCTCAGCACTCCGAAAGTAGAGGCAGGTG(+AAAAAA)GATCCCTGAGTTTATGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10