Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165210_op08 GCTGACTTCAGACACACCAGAAGAAATCATCG(+AAAAAA)GATCCCATTACGAATGTTTGGGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606165208_a3bt ACCACATGGTGGCTCCCAAACATTCGTAATGG(+AAAAAA)GATCCGATGATTTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606165207_cnil GGAAAGACCATCCAGAGACTGCCTCACATGGG(+AAAAAA)GATCCATCCCATATACAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606165205_t2os AGTGTCTGGGTTTGGTGACTGTATATGGGATG(+AAAAAA)GATCCCCATGTGAGGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606165204_94wz GAGGCTGTCTCAGTCTCTGTTGCCTATGATTG(+AAAAAA)GATCCCCTTCTGCTTGCTGGACTTCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606165202_ywxc GGCCCACCCAGGAAGTCCAGCAAGCAGAAGGG(+AAAAAA)GATCCAATCATAGGCAACAGAGACTGAGACA NGG 10 8 17 8 24 0 100 5 10
    240606165159_nzpy TGGGTTTGTTTTCCCTGAACCCCCTCCCCCAG(+AAAAAA)GATCCAGACTTCCCACTTATATAGCTTATCA NGG 10 8 17 8 24 0 100 5 10
    240606165157_rnrb ATAGTTGATAAGCTATATAAGTGGGAAGTCTG(+AAAAAA)GATCCTGGGGGAGGGGGTTCAGGGAAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606165155_94j8 CATGTCTGTCAGGCATTTATATAAATTTTGGG(+AAAAAA)GATCCAAACTCTGGATTTTATTGTTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240606165154_0acu AGCACAAAAATAACAATAAAATCCAGAGTTTG(+AAAAAA)GATCCCCAAAATTTATATAAATGCCTGACAG NGG 10 8 17 8 24 0 100 5 10