Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170400_5uzb GAAATGACAAGAGGGAAAACAGCTGGTGGCGG(+AAAAAA)GATCCCAGTCAGCCTTCCCGCTCTGCACCTG NGG 10 8 17 8 24 0 100 5 10
    240606170359_k3ms GGGTGGCCAGGAGCAGCAGAGGTGAAGGGATG(+AAAAAA)GATCCACCTGCGGGTCTCTGTGGTTACCGTA NGG 10 8 17 8 24 0 100 5 10
    240606170357_h620 ATCACTACGGTAACCACAGAGACCCGCAGGTG(+AAAAAA)GATCCATCCCTTCACCTCTGCTGCTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606170356_2kk5 CTCTGCATCCATCTTGATTAAAGATGGGTGTG(+AAAAAA)GATCCTCTCCCACATCCTAGGGTGTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240606170355_ylue ATGGCTGCCCCACACCCTAGGATGTGGGAGAG(+AAAAAA)GATCCACACCCATCTTTAATCAAGATGGATG NGG 10 8 17 8 24 0 100 5 10
    240606170354_k7wt CTCCCTTCAGCACGGTAGGTCTTTGCATCTAG(+AAAAAA)GATCCCCGCCCCAGACCGCCTGTGAGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606170352_27ru CTTTGCTCTGCTCACAGGCGGTCTGGGGCGGG(+AAAAAA)GATCCTAGATGCAAAGACCTACCGTGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606170351_0f1q TGGTAGCCTGTGGTCAGGCAGCCCTTGTAATG(+AAAAAA)GATCCCTACCTTCTTGTAAAGTCTCTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606170349_u6wf ACACATTTTTAGAGACTTTACAAGAAGGTAGG(+AAAAAA)GATCCATTACAAGGGCTGCCTGACCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240606170348_qe31 AATACCCTCTTTCTGACCTCCACAGGCACAGG(+AAAAAA)GTACCCATGTGGTATACATATATACACAAGG NGG 10 8 17 8 24 0 100 5 10