Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165121_xnuu ATACTCCCAGAATGAGCAGAGGGTCCAAAGTG(+AAAAAA)GATCCCTGAGCCATTTTGAATAATTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240606165120_a18y AGTGGTGATAAAATTATTCAAAATGGCTCAGG(+AAAAAA)GATCCACTTTGGACCCTCTGCTCATTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606165118_b23r TAGCACAGGGTCCAGGCAACCTCAGCCTATGG(+AAAAAA)GATCCTTAGGCACTGTGTCACAGGAACGTCC NGG 10 8 17 8 24 0 100 5 10
    240606165116_bqc2 AGCTAGGACGTTCCTGTGACACAGTGCCTAAG(+AAAAAA)GATCCCATAGGCTGAGGTTGCCTGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240606165115_a27z GTTTCAAGATTTTCTAACACAAATTTACATTG(+AAAAAA)GTACCAAAATGGGCAAGAAAATGAAGGCACA NGG 10 8 17 8 24 0 100 5 10
    240606165113_egzv GAGCCTGTGCCTTCATTTTCTTGCCCATTTTG(+AAAAAA)GTACCAATGTAAATTTGTGTTAGAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240606165111_hj9q CCAAAAAATACAGCAAAGACAGGAACGTCCAG(+AAAAAA)GATCCGGGTCTGTTAATAAAGCCACCTCGAG NGG 10 8 17 8 24 0 100 5 10
    240606165109_p9lc CTAGTCTCGAGGTGGCTTTATTAACAGACCCG(+AAAAAA)GATCCTGGACGTTCCTGTCTTTGCTGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606165107_tiqf ATCAGGCTGGGGAGGTTAGTGCACAATCAGTG(+AAAAAA)GATCCATGCCCCGCCTCCAAGCTCTGCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606165106_mhp9 GTGGGGTTTGCAGAGCTTGGAGGCGGGGCATG(+AAAAAA)GATCCACTGATTGTGCACTAACCTCCCCAGC NGG 10 8 17 8 24 0 100 5 10