Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165037_1wbl CTTAGTCTGGGGTAAAGAGGCTGGTGAAGTAG(+AAAAAA)GTACCTCCAATTGCAGTGTGGTCACTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240606165035_bbtz ATGGTATTAAAGTGACCACACTGCAATTGGAG(+AAAAAA)GTACCTACTTCACCAGCCTCTTTACCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606165034_bjd8 TGAAATTGTCAATTTCAGAGCATGAAGTGAAG(+AAAAAA)GTACCGACTCAAGAAAAAGATTTTTACTGCA NGG 10 8 17 8 24 0 100 5 10
    240606165033_uxyn ACTGATGCAGTAAAAATCTTTTTCTTGAGTCG(+AAAAAA)GTACCTTCACTTCATGCTCTGAAATTGACAA NGG 10 8 17 8 24 0 100 5 10
    240606165031_hq42 GGTTATAGTTTCAGTGTAGCTCTGGGAACAGG(+AAAAAA)GATCCCTGGACCACTAATACTTTCTAGATCT NGG 10 8 17 8 24 0 100 5 10
    240606165029_yd7j TAGATAGATCTAGAAAGTATTAGTGGTCCAGG(+AAAAAA)GATCCCTGTTCCCAGAGCTACACTGAAACTA NGG 10 8 17 8 24 0 100 5 10
    240606165028_2qgs AATTTTCCATTCCTGCATTAGTCTAGTGTATG(+AAAAAA)GATCCACGTGCTCTCTTTTAGTATTATCCTA NGG 10 8 17 8 24 0 100 5 10
    240606165027_dzes ACTTATAGGATAATACTAAAAGAGAGCACGTG(+AAAAAA)GATCCATACACTAGACTAATGCAGGAATGGA NGG 10 8 17 8 24 0 100 5 10
    240606165026_gdq5 TAAATTCAATATAAATTGTGATCTCTTGCTGG(+AAAAAA)GATCCCAGGCCCATTGTGTTTCCAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606165023_qgnv TCCCTACAGCCCTGGAAACACAATGGGCCTGG(+AAAAAA)GATCCCAGCAAGAGATCACAATTTATATTGA NGG 10 8 17 8 24 0 100 5 10