Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165006_a1g6 GCTGGTCTAAGAGAAGTGCGGAAGCAAAATGG(+AAAAAA)GATCCTTAAGCCAGCTGTGGTGGCTCAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606165004_776j TAATCAACCTGAGCCACCACAGCTGGCTTAAG(+AAAAAA)GATCCCATTTTGCTTCCGCACTTCTCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240606165003_h1v6 CCCTAAATATAGGATGGGAATCCTTGGGATAG(+AAAAAA)GATCCTGAGTTAAGTAAGGGGGTGAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606165001_i4ru TCAGCTTGCTTTCACCCCCTTACTTAACTCAG(+AAAAAA)GATCCTATCCCAAGGATTCCCATCCTATATT NGG 10 8 17 8 24 0 100 5 10
    240606165000_bs3x GATTCACTACAGTGTGAGCAACTCGGCTCTTG(+AAAAAA)GATCCAAAGCAGCACTTCATATATAAAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606164959_nkzd GTTTGCCCTTTTATATATGAAGTGCTGCTTTG(+AAAAAA)GATCCAAGAGCCGAGTTGCTCACACTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606164958_58wf GACCCACAGGAACTATGAAGGAAGAAGCGTAG(+AAAAAA)GATCCCAGAGCAATCATATAACCGAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240606164956_n46g CGGTTCAGAGTTCGGTTATATGATTGCTCTGG(+AAAAAA)GATCCTACGCTTCTTCCTTCATAGTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606164955_iv29 TCTTCGCCCTACTAAGCCATTCCCATATGTAG(+AAAAAA)GATCCTTCTTCTTGTCAGAAGATCAAATTAC NGG 10 8 17 8 24 0 100 5 10
    240606164953_ihoc TTTAAGTAATTTGATCTTCTGACAAGAAGAAG(+AAAAAA)GATCCTACATATGGGAATGGCTTAGTAGGGC NGG 10 8 17 8 24 0 100 5 10