Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164952_v2rm AAGGAGAGTGTTCAGGCAATATAATTGATCTG(+AAAAAA)GTACCAGGGTTCAAGGGTGAATGGTGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606164950_jf8w TCTTTGCACCCACCATTCACCCTTGAACCCTG(+AAAAAA)GTACCAGATCAATTATATTGCCTGAACACTC NGG 10 8 17 8 24 0 100 5 10
    240606164948_h8zd AGGGGAGGGTTAGTTCTATCACCTTTTTTATG(+AAAAAA)GTACCTGATTGCTTTGCTTATCCATCAACAG NGG 10 8 17 8 24 0 100 5 10
    240606164947_1elz GAGCACTGTTGATGGATAAGCAAAGCAATCAG(+AAAAAA)GTACCATAAAAAAGGTGATAGAACTAACCCT NGG 10 8 17 8 24 0 100 5 10
    240606164946_sl2z AGGGGAGGGTTAGTTCTATCACCTTTTTTATG(+AAAAAA)GTACCTGATTGCTTTGCTTATCCATCAACAG NGG 10 8 17 8 24 0 100 5 10
    240606164945_sel3 GAGCACTGTTGATGGATAAGCAAAGCAATCAG(+AAAAAA)GTACCATAAAAAAGGTGATAGAACTAACCCT NGG 10 8 17 8 24 0 100 5 10
    240606164943_wbkt GTTGCCTGCTTCTGAGAAAGTTATGTGTGTAG(+AAAAAA)GTACCATGTAAAGGATTTGGCTACAGATGTA NGG 10 8 17 8 24 0 100 5 10
    240606164942_5p02 ACCAATACATCTGTAGCCAAATCCTTTACATG(+AAAAAA)GTACCTACACACATAACTTTCTCAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606164941_7d7v TTCAGGATTTTCAGTCTTGCTGGCAATAGACG(+AAAAAA)GATCCGAAACCCTAAGTACAGGGATCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606164939_y2el CCTTCTTTCAGATCCCTGTACTTAGGGTTTCG(+AAAAAA)GATCCGTCTATTGCCAGCAAGACTGAAAATC NGG 10 8 17 8 24 0 100 5 10