Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170127_m974 ATTGTGAAGAGAGTGAAAGTAAACCCGAAGGG(+AAAAAA)GATCCTCACGTGCTAAATAGATGGCGGGCGG NGG 10 8 17 8 24 0 100 5 10
    240606170126_oqjf CTAGCTCCAGACCCTTCTCTACAGATGTTGTG(+AAAAAA)GTACCCTCCAGCCTCCAAGCCACACAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240606170124_8m5y TGTGCCTGACTGTGTGGCTTGGAGGCTGGAGG(+AAAAAA)GTACCACAACATCTGTAGAGAAGGGTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606170121_un1t TTCCCCACTTCCTCACAAAGCCCTGACCCCAG(+AAAAAA)GATCCCAGCGATGGACAGGAACACCTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606170119_43mb GTAGACTCTGAGGTGTTCCTGTCCATCGCTGG(+AAAAAA)GATCCTGGGGTCAGGGCTTTGTGAGGAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606170118_bxem TGGCATGGTCATGTGCCCTCTGGCCTGTGGAG(+AAAAAA)GTACCTATAGTGGCAAGGACAGGGGTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240606170116_g98l CTCCCACCTGACCCCTGTCCTTGCCACTATAG(+AAAAAA)GTACCTCCACAGGCCAGAGGGCACATGACCA NGG 10 8 17 8 24 0 100 5 10
    240606170115_7t41 CCTGGAACAGGATATCAATGAAGCAGCTGAGG(+AAAAAA)GTACCAAGAGTGTGGGGTAAGGATTTCATTC NGG 10 8 17 8 24 0 100 5 10
    240606170113_fg70 CCCTTGAATGAAATCCTTACCCCACACTCTTG(+AAAAAA)GTACCCTCAGCTGCTTCATTGATATCCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606170111_0xts CTGAGGAAGCTACAAAGTGTGTTCTCTCCAGG(+AAAAAA)GTACCTTCCAATGCTTCCCTAGCTTCTTGGC NGG 10 8 17 8 24 0 100 5 10