Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164806_0qxv GAGGCTTCAGCGAGCATCGATGTCTGCCTCAG(+AAAAAA)GATCCTTACATCCCTGCATAGCATCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240606164805_jseb GTGTACACACTGATGCTATGCAGGGATGTAAG(+AAAAAA)GATCCTGAGGCAGACATCGATGCTCGCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606164803_w0fj GGTTACCTACCCACCACCCCTCCAGGCACCAG(+AAAAAA)GATCCCCTATTTAGCTTCCTGTAACGCCCCC NGG 10 8 17 8 24 0 100 5 10
    240606164801_kxis GCTGTGGGGGCGTTACAGGAAGCTAAATAGGG(+AAAAAA)GATCCTGGTGCCTGGAGGGGTGGTGGGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606164759_ukae GCTTTGGTATAGTGAATGTCTCCCTGTGCAGG(+AAAAAA)GTACCACGCCATGTGCCATTTAGGCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240606164758_ov4q GTCTGGCTTGTGCCTAAATGGCACATGGCGTG(+AAAAAA)GTACCCTGCACAGGGAGACATTCACTATACC NGG 10 8 17 8 24 0 100 5 10
    240606164757_hrxp ATATATCCAGTCAAGGAGCAATCACACTTTGG(+AAAAAA)GATCCGCATCTCAAAGATAGGGTGAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606164755_yuva CATCTCCTCTCTCACCCTATCTTTGAGATGCG(+AAAAAA)GATCCCAAAGTGTGATTGCTCCTTGACTGGA NGG 10 8 17 8 24 0 100 5 10
    240606164753_4cm9 AAAGGAAAGGTGTCTCTCCTACGGGCCAGGGG(+AAAAAA)GATCCTATCACAAAAGAATAAAGCAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606164752_eybh TCCAATCAGGCTGCTTTATTCTTTTGTGATAG(+AAAAAA)GATCCCCCTGGCCCGTAGGAGAGACACCTTT NGG 10 8 17 8 24 0 100 5 10