Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164716_vba3 ACAGTATCTAAAGGTAGTTATTTCTCCTTCTG(+AAAAAA)GATCCGGTGCAGCATACCTATGATTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606164714_t8po TCTAGAGCTGGAATCATAGGTATGCTGCACCG(+AAAAAA)GATCCAGAAGGAGAAATAACTACCTTTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606164712_fgga TTTGATAGTAAGATTATAAGGTGGCTCCAGAG(+AAAAAA)GTACCTTGGATAGGACTGCGCCCTGATAAGA NGG 10 8 17 8 24 0 100 5 10
    240606164710_57ev TTTCATCTTATCAGGGCGCAGTCCTATCCAAG(+AAAAAA)GTACCTCTGGAGCCACCTTATAATCTTACTA NGG 10 8 17 8 24 0 100 5 10
    240606164708_eu99 GCGCCAAGTGGAACGTGGAGAGTGGAGGCCTG(+AAAAAA)GTACCTGAGGTTTTAGTGGTAACCAAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606164706_rfgq TAGATAGTCCTTGGTTACCACTAAAACCTCAG(+AAAAAA)GTACCAGGCCTCCACTCTCCACGTTCCACTT NGG 10 8 17 8 24 0 100 5 10
    240606164705_bosp GGGGACATTGTTAAATAGGGAAGGTGATGGTG(+AAAAAA)GATCCTGACTGCTCCTACCCTGTGACCTTTA NGG 10 8 17 8 24 0 100 5 10
    240606164703_3rjg ATGACTAAAGGTCACAGGGTAGGAGCAGTCAG(+AAAAAA)GATCCACCATCACCTTCCCTATTTAACAATG NGG 10 8 17 8 24 0 100 5 10
    240606164702_h1yq ACAAGCACCCCTAAACCACCTCTGCCTACAGG(+AAAAAA)GATCCCCAGGACCCCAATGGGCATCAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606164659_8lsu GTTCAAAGGCTGATGCCCATTGGGGTCCTGGG(+AAAAAA)GATCCCTGTAGGCAGAGGTGGTTTAGGGGTG NGG 10 8 17 8 24 0 100 5 10