Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164658_rwdq CCTCAGGGTCAATGTTATACTCGGGTGTTGTG(+AAAAAA)GATCCACCTGGGCCAGTGCATCTACATCTTC NGG 10 8 17 8 24 0 100 5 10
    240606164656_e05y CAGAAGAAGATGTAGATGCACTGGCCCAGGTG(+AAAAAA)GATCCACAACACCCGAGTATAACATTGACCC NGG 10 8 17 8 24 0 100 5 10
    240606164655_iqqt GATTGTGGTTCAGTTTGTCTAATGTCAACTTG(+AAAAAA)GTACCTACAGTAAAACTGTAAGTCCACTCGA NGG 10 8 17 8 24 0 100 5 10
    240606164654_499m ATTTGTCGAGTGGACTTACAGTTTTACTGTAG(+AAAAAA)GTACCAAGTTGACATTAGACAAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240606164653_v11w CAGGTTTCATTTTAAGAAAGTCTTCCATGCTG(+AAAAAA)GTACCCTGTTTTGTTCAATTTTTTTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606164651_2fab AGTTTATGCAGAAAAAAATTGAACAAAACAGG(+AAAAAA)GTACCAGCATGGAAGACTTTCTTAAAATGAA NGG 10 8 17 8 24 0 100 5 10
    240606164650_e3ep TTCTTTGGCTTGTATAGTTGCGAATCTAGAAG(+AAAAAA)GATCCACAATACAAAGTTTTGATGGGATAAT NGG 10 8 17 8 24 0 100 5 10
    240606164648_vies GAGAAATTATCCCATCAAAACTTTGTATTGTG(+AAAAAA)GATCCTTCTAGATTCGCAACTATACAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606164647_gsyo AGAAAACACATTTGAGCGACGCACAGAGGGTG(+AAAAAA)GATCCCTAAAGTCTTGTTCTGGGCCTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606164645_v83r ACCAGAGCAGAGGCCCAGAACAAGACTTTAGG(+AAAAAA)GATCCACCCTCTGTGCGTCGCTCAAATGTGT NGG 10 8 17 8 24 0 100 5 10