Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164556_yhtl TGTGCACTATCCATGTGACCGAAGGACACATG(+AAAAAA)GATCCTACCGCTAAGCCCTTCCTGATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606164554_x0ix TGGCCTGGGCATCAGGAAGGGCTTAGCGGTAG(+AAAAAA)GATCCATGTGTCCTTCGGTCACATGGATAGT NGG 10 8 17 8 24 0 100 5 10
    240606164553_4obt ATCTTCTGAATCCCTGTTTACAAATGCTGCAG(+AAAAAA)GATCCTTTCATGAATCAGAATGAGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240606164552_vkmg CCCTCATCTGTGCTCATTCTGATTCATGAAAG(+AAAAAA)GATCCTGCAGCATTTGTAAACAGGGATTCAG NGG 10 8 17 8 24 0 100 5 10
    240606164551_uhg1 AGTTTCCTAAGCAACTCTAGAAGTGCCACTTG(+AAAAAA)GATCCATGGAATTACACTGACTTCTCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606164549_u8w1 GTAAAGGGAAGAGAAGTCAGTGTAATTCCATG(+AAAAAA)GATCCAAGTGGCACTTCTAGAGTTGCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240606164547_tt55 TTTTACTCCCAAGACCTCAGGGGGAGTAACTG(+AAAAAA)GTACCCAAAGTACCACTTGGTCTGAGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606164546_ioit AGAGAGAAGGCTCAGACCAAGTGGTACTTTGG(+AAAAAA)GTACCAGTTACTCCCCCTGAGGTCTTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606164544_1aay AGTCTTGGGAGAGGCTCAGGATGGAATTTTGG(+AAAAAA)GATCCCTGGGGTCTTTGGTACGGAGGATAAG NGG 10 8 17 8 24 0 100 5 10
    240606164541_9stt GTCACCTTATCCTCCGTACCAAAGACCCCAGG(+AAAAAA)GATCCCAAAATTCCATCCTGAGCCTCTCCCA NGG 10 8 17 8 24 0 100 5 10