Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164456_nsd3 CCTGTTCTGAATATTTCATACAGACGTGACTG(+AAAAAA)GATCCTTTTAACTGGCCAGTGATTTCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606164455_ibty GAATATCCTTGAAATCACTGGCCAGTTAAAAG(+AAAAAA)GATCCAGTCACGTCTGTATGAAATATTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606164453_iuh9 ATTTCCTGCCCTTGGGACTATTTAGACAAGGG(+AAAAAA)GATCCCAAAACTGGGCAGAACAAATGAACCA NGG 10 8 17 8 24 0 100 5 10
    240606164451_bxzv CCTGGTGGTTCATTTGTTCTGCCCAGTTTTGG(+AAAAAA)GATCCCCTTGTCTAAATAGTCCCAAGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606164450_jm24 AGGCAGGGGAAGGATTTATCAAGAGATTCTTG(+AAAAAA)GTACCAGCAGTAGCGACCACATGCACTGAGT NGG 10 8 17 8 24 0 100 5 10
    240606164449_ok5w GCTCAACTCAGTGCATGTGGTCGCTACTGCTG(+AAAAAA)GTACCAAGAATCTCTTGATAAATCCTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240606164447_b63o GTGAAGGAATCACTCCTGGTGCAATACTGTGG(+AAAAAA)GTACCAAGGCCTGTCTAAGCCACTTCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606164445_xh1f GATCCCTCCTGAAGTGGCTTAGACAGGCCTTG(+AAAAAA)GTACCCACAGTATTGCACCAGGAGTGATTCC NGG 10 8 17 8 24 0 100 5 10
    240606164443_whaq CACGCCGGCTCCTGTTTAGAAAGCGGGTGGGG(+AAAAAA)GATCCACAGAACACACATGCAGAATGCATCC NGG 10 8 17 8 24 0 100 5 10
    240606164442_x1au GATCAGGATGCATTCTGCATGTGTGTTCTGTG(+AAAAAA)GATCCCCCACCCGCTTTCTAAACAGGAGCCG NGG 10 8 17 8 24 0 100 5 10