Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164424_ncrc TATGAAAATGGAGGGTAGACAACAGGTATCAG(+AAAAAA)GTACCCACAAAATGAACCTCACCATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240606164423_pp2u TGTGGTACTTCATGGTGAGGTTCATTTTGTGG(+AAAAAA)GTACCTGATACCTGTTGTCTACCCTCCATTT NGG 10 8 17 8 24 0 100 5 10
    240606164421_wt1u GTTCTGAAGTAGATGGTTGCCATGAACTCCCG(+AAAAAA)GATCCAGATAGTCTCATTCATGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606164419_36aa GGGATCACCTCCATCATGAATGAGACTATCTG(+AAAAAA)GATCCGGGAGTTCATGGCAACCATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240606164418_v2ye AATGAAAAGCAGCTAACTACAGAAGCAACATG(+AAAAAA)GATCCTGCTCTGATTACCCATCGTCCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240606164417_3q93 TCAGCTCTAGGGACGATGGGTAATCAGAGCAG(+AAAAAA)GATCCATGTTGCTTCTGTAGTTAGCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240606164415_6msv CAGGATGAGAACAGGCTGGGATGAACTCAGAG(+AAAAAA)GTACCAGATGAGCTTGATCTTGAAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606164414_zvnx TGAGTCAGACTCTTCAAGATCAAGCTCATCTG(+AAAAAA)GTACCTCTGAGTTCATCCCAGCCTGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606164413_ufkx GTTTCACAACGCTGAAAATAAGGTGGCAGGTG(+AAAAAA)GTACCCTTCCTTCCCAGAAGGAAAGAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606164411_ubcz TCTTCTTCTCTCTTTCCTTCTGGGAAGGAAGG(+AAAAAA)GTACCACCTGCCACCTTATTTTCAGCGTTGT NGG 10 8 17 8 24 0 100 5 10