Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165538_iejg CTATATCTTTTCCCATTGACTCTGGATTAAAG(+AAAAAA)GTACCAAAGCTAAAGAACAGAATGAAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606165537_9xmx TGCACATGCCCTTTTCTGTCCAGTTTAGGTTG(+AAAAAA)GATCCTTAGTACCTAGACACACTCTCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606165535_n4zr CACGTAAGAGGAGAGTGTGTCTAGGTACTAAG(+AAAAAA)GATCCAACCTAAACTGGACAGAAAAGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240606165534_w65f TGTGTCCCCAATCCCATCCATCTGTCTGAAAG(+AAAAAA)GATCCCAGCATCCCTAGGCTTGGCATCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606165533_9ngd CCTTCCTCAGATGCCAAGCCTAGGGATGCTGG(+AAAAAA)GATCCTTTCAGACAGATGGATGGGATTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606165531_tlx4 AAGTTTAAATAATCTAAGTTTCTTTGCAGCTG(+AAAAAA)GATCCAACTGCTAGAAGGCAATTCTTAGAGC NGG 10 8 17 8 24 0 100 5 10
    240606165530_1kev AGATTGCTCTAAGAATTGCCTTCTAGCAGTTG(+AAAAAA)GATCCAGCTGCAAAGAAACTTAGATTATTTA NGG 10 8 17 8 24 0 100 5 10
    240606165528_4qzo GGTTGAAGGAAGAAGACCTACTCTGAACTTGG(+AAAAAA)GTACCAATATCCCATGGGCAGAACAAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606165527_3pvp TTTATTCTTTTTGTTCTGCCCATGGGATATTG(+AAAAAA)GTACCCAAGTTCAGAGTAGGTCTTCTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606165526_1pvt CCCTAAACAGGGCAGCAGAATATAGTTTAGTG(+AAAAAA)GTACCCAGCTATAGAAATAAATCTTAGGGGT NGG 10 8 17 8 24 0 100 5 10