Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164218_r3fo ATGAGATGAAATATTTGCTTCGCACACGCAGG(+AAAAAA)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606164216_nd00 AAGGTCTCCTTTGAAGAGAACTTCTATAGTGG(+AAAAAA)GATCCCTGCGTGTGCGAAGCAAATATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606164215_2hj4 TGTTAGGAGTAGGTTCCTGGGCCTGGAAACAG(+AAAAAA)GATCCTGGTTCAACCCTTCCAGCAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240606164213_vy42 GCTTCTCCATCTTGCTGGAAGGGTTGAACCAG(+AAAAAA)GATCCTGTTTCCAGGCCCAGGAACCTACTCC NGG 10 8 17 8 24 0 100 5 10
    240606164211_00pp CCTTGATGCAGTCTGTGCCTGACCGCTGCCTG(+AAAAAA)GTACCAACAGTAACACTACAAAAGGCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240606164210_5nez AGTCCAGAGAGCCTTTTGTAGTGTTACTGTTG(+AAAAAA)GTACCAGGCAGCGGTCAGGCACAGACTGCAT NGG 10 8 17 8 24 0 100 5 10
    240606164208_ijc4 AGGGTAAATAGGCAACATTGCCCCTAAGCCTG(+AAAAAA)GATCCTAAAGATGGTTCTAGCCATGCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606164207_no3h TACAGGAAGGGCATGGCTAGAACCATCTTTAG(+AAAAAA)GATCCAGGCTTAGGGGCAATGTTGCCTATTT NGG 10 8 17 8 24 0 100 5 10
    240606164206_ri64 GTTTTCCTAAAGAACTTCTCACATCTCAAGAG(+AAAAAA)GATCCCGGTTGAGCATCATTTGTGTAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606164203_zem2 CATCACCACCTACACAAATGATGCTCAACCGG(+AAAAAA)GATCCTCTTGAGATGTGAGAAGTTCTTTAGG NGG 10 8 17 8 24 0 100 5 10