Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164115_09rm GATCACTAATGACAGGTCGCTTTCCTCCAGGG(+AAAAAA)GTACCACTGAGCTACAGCAGGACATCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606164114_ibx6 GCTCGGCCATGATGTCCTGCTGTAGCTCAGTG(+AAAAAA)GTACCCCTGGAGGAAAGCGACCTGTCATTAG NGG 10 8 17 8 24 0 100 5 10
    240606164113_ajn2 CCTACACCCAACCACTCTACACTGAATAGATG(+AAAAAA)GATCCTCCATCAAAGAATTGTCTTTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240606164112_qnth GACATTAAAAGAAAGACAATTCTTTGATGGAG(+AAAAAA)GATCCATCTATTCAGTGTAGAGTGGTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606164111_mre2 TTCGAAGGCTAACATGAGAGATGTTACAGATG(+AAAAAA)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240606164109_6jl6 TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+AAAAAA)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606164108_b67c TTCCAAGGCTAACATGAGAGATGTTACAGATG(+AAAAAA)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240606164107_1t6x TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+AAAAAA)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606164106_wx83 TTCCAAGGCTAACATGAGAGATGTTACAGATG(+AAAAAA)GTACCTCAAAGTTTTTCAAATTCACTTGACT NGG 10 8 17 8 24 0 100 5 10
    240606164104_r1ls TGATCAGTCAAGTGAATTTGAAAAACTTTGAG(+AAAAAA)GTACCATCTGTAACATCTCTCATGTTAGCCT NGG 10 8 17 8 24 0 100 5 10