Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164103_obew ATGTATGTTTTGCCTGCCACAACCCGTACCTG(+AAAAAA)GTACCAGCAAAACCTGCAAAAAGCAAACAAG NGG 10 8 17 8 24 0 100 5 10
    240606164101_c3v9 CCTGGCTTGTTTGCTTTTTGCAGGTTTTGCTG(+AAAAAA)GTACCAGGTACGGGTTGTGGCAGGCAAAACA NGG 10 8 17 8 24 0 100 5 10
    240606164100_w51l AGACTCATTAATAGGATTGTTAGCAACCACTG(+AAAAAA)GATCCTCTGGTGGATAGACAAATGTTCTATA NGG 10 8 17 8 24 0 100 5 10
    240606164058_rnl8 TTCCATATAGAACATTTGTCTATCCACCAGAG(+AAAAAA)GATCCAGTGGTTGCTAACAATCCTATTAATG NGG 10 8 17 8 24 0 100 5 10
    240606164056_tqwh ATGAGATGAAATATTTGCTTCGCACACGCAGG(+AAAAAA)GATCCCACTATAGAAGTTCTCTTCAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606164054_m6a3 AAGGTCTCCTTTGAAGAGAACTTCTATAGTGG(+AAAAAA)GATCCCTGCGTGTGCGAAGCAAATATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240606164053_juo9 AAGTGAAGGCTATGATTATTTCTCTAGTGCAG(+AAAAAA)GATCCGGACAGGGTTCCACAAATCAATTCCT NGG 10 8 17 8 24 0 100 5 10
    240606164052_7m6r GATATAGGAATTGATTTGTGGAACCCTGTCCG(+AAAAAA)GATCCTGCACTAGAGAAATAATCATAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240606164051_5bo2 ATCCCAGAAAAATAGGAAGTCATTATAAATAG(+AAAAAA)GATCCAATGGAACAATGTTCTTCAAATAATA NGG 10 8 17 8 24 0 100 5 10
    240606164049_vx3h AGTTATATTATTTGAAGAACATTGTTCCATTG(+AAAAAA)GATCCTATTTATAATGACTTCCTATTTTTCT NGG 10 8 17 8 24 0 100 5 10