Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164146_vfvu TAAAATAATGAGACAGTTTTTTGTTATTGGTG(+AAAAAA)GATCCAGGAGCTGTTTAGAAACTATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240606164145_uh48 GGGGAAAATACATAGTTTCTAAACAGCTCCTG(+AAAAAA)GATCCACCAATAACAAAAAACTGTCTCATTA NGG 10 8 17 8 24 0 100 5 10
    240606164143_u6yt TGGGGCCCAGCTGCGGCTGGCTCCCGGCCTGG(+AAAAAA)GTACCAGAAAGAGACCTTCAGCTCAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606164141_qu4a CCAGCCTGGGTTGAGCTGAAGGTCTCTTTCTG(+AAAAAA)GTACCCAGGCCGGGAGCCAGCCGCAGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606164140_z5bl TCTGTTGTTACTAATAACAGACTTTATCATGG(+AAAAAA)GTACCAGCCATAGCGAGTTAATAAGTGACAT NGG 10 8 17 8 24 0 100 5 10
    240606164138_1srp TGGATATGTCACTTATTAACTCGCTATGGCTG(+AAAAAA)GTACCCATGATAAAGTCTGTTATTAGTAACA NGG 10 8 17 8 24 0 100 5 10
    240606164137_l1t6 TTCGACATTTTAACCTGTTCTGCCTGACATGG(+AAAAAA)GATCCTCCATCCCAGCTCCTTCCCCAACACT NGG 10 8 17 8 24 0 100 5 10
    240606164135_kvnq AGCTCAGTGTTGGGGAAGGAGCTGGGATGGAG(+AAAAAA)GATCCCATGTCAGGCAGAACAGGTTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240606164133_noxl TTGATGCCTTCTTTTAAAGTACCCTGCTCCTG(+AAAAAA)GTACCCAGTTAATCCCCTCTCCCAGTATAAA NGG 10 8 17 8 24 0 100 5 10
    240606164132_x8po CCGACTTTATACTGGGAGAGGGGATTAACTGG(+AAAAAA)GTACCAGGAGCAGGGTACTTTAAAAGAAGGC NGG 10 8 17 8 24 0 100 5 10