Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164234_9ru0 AGACAGGTTGCTGAGGCTGGCTTTGAACTTGG(+AAAAAA)GATCCTGAGGCTTCAGACTCCCAGGTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606164232_1aci ATAAACAGCCACCTGGGAGTCTGAAGCCTCAG(+AAAAAA)GATCCCAAGTTCAAAGCCAGCCTCAGCAACC NGG 10 8 17 8 24 0 100 5 10
    240606164230_i39h TTTGTAGTCTCGGGTGAATTAGGCAGGACCCG(+AAAAAA)GATCCAAGGGAGACCTGGAGCACAACCGACG NGG 10 8 17 8 24 0 100 5 10
    240606164228_tz0o ATCCGCGTCGGTTGTGCTCCAGGTCTCCCTTG(+AAAAAA)GATCCGGGTCCTGCCTAATTCACCCGAGACT NGG 10 8 17 8 24 0 100 5 10
    240606164227_34gi TCCTGTTGAATGACCCTCATTGCCTTGGTGTG(+AAAAAA)GATCCCAGCCCTCACCTCCTGCAACACTAAT NGG 10 8 17 8 24 0 100 5 10
    240606164225_syls CTTAAATTAGTGTTGCAGGAGGTGAGGGCTGG(+AAAAAA)GATCCACACCAAGGCAATGAGGGTCATTCAA NGG 10 8 17 8 24 0 100 5 10
    240606164223_jpv4 TTATTAAAATTTCACACAAACACAAGCACAGG(+AAAAAA)GTACCAGAGCTCTCTTCCTGGATACCAGGTA NGG 10 8 17 8 24 0 100 5 10
    240606164222_zawn AAAGCTACCTGGTATCCAGGAAGAGAGCTCTG(+AAAAAA)GTACCCTGTGCTTGTGTTTGTGTGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240606164221_6et8 CTTTTGATGTGGATAATGTGTCAAACTATGTG(+AAAAAA)GATCCCAATGTCAGGATGAAAAAAAGTATCC NGG 10 8 17 8 24 0 100 5 10
    240606164219_aj3q AGCATGGATACTTTTTTTCATCCTGACATTGG(+AAAAAA)GATCCACATAGTTTGACACATTATCCACATC NGG 10 8 17 8 24 0 100 5 10