Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165524_3g9c CTCTGACCCCTAAGATTTATTTCTATAGCTGG(+AAAAAA)GTACCACTAAACTATATTCTGCTGCCCTGTT NGG 10 8 17 8 24 0 100 5 10
    240606165523_p4ma CAAAGCATGCTTACTTGATGCTTTATAAGTGG(+AAAAAA)GATCCAGGATGGGGAATGCAAATTAGTTATA NGG 10 8 17 8 24 0 100 5 10
    240606165521_hax2 ATATTTATAACTAATTTGCATTCCCCATCCTG(+AAAAAA)GATCCCACTTATAAAGCATCAAGTAAGCATG NGG 10 8 17 8 24 0 100 5 10
    240606165519_wsle GTATGTCTAGTTCCTCTCACTTGACCCTAGGG(+AAAAAA)GATCCTAATCCTTGCCATTTCATAGATATTT NGG 10 8 17 8 24 0 100 5 10
    240606165518_5o7n CTTTGAAATATCTATGAAATGGCAAGGATTAG(+AAAAAA)GATCCCCTAGGGTCAAGTGAGAGGAACTAGA NGG 10 8 17 8 24 0 100 5 10
    240606165515_a4tz AGCAATTGCTTCCGTAGTGTCTGGACATCCGG(+AAAAAA)GTACCCGGGCATCAAATCCAGGAGCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240606165513_zi8q AGGCACCTTTGGCTCCTGGATTTGATGCCCGG(+AAAAAA)GTACCCGGATGTCCAGACACTACGGAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240606165511_26gq GAAATGTTTAAATTAACTGTGTTGTACAAATG(+AAAAAA)GTACCCAACACAAAACCTTTTTTAAATTAGT NGG 10 8 17 8 24 0 100 5 10
    240606165510_bane GTCTTACTAATTTAAAAAAGGTTTTGTGTTGG(+AAAAAA)GTACCATTTGTACAACACAGTTAATTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240606165508_3680 TGGTGGGCTCAGGTTTGCTTGGTCTTGTGTGG(+AAAAAA)GTACCCATTGCCACTTTGAGTTTATGGGTTT NGG 10 8 17 8 24 0 100 5 10