Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164338_peeg ATCCACCATGTGGGTGCTGGAATCGAACCCAG(+AAAAAA)GTACCCTGGAAGAGCAGCCACTATTCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240606164336_3l4y CAGCAGTTAAGAATAGTGGCTGCTCTTCCAGG(+AAAAAA)GTACCTGGGTTCGATTCCAGCACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240606164335_1h03 GCACTTGCCACCAAGTCTAGAGACTTGAGTTG(+AAAAAA)GATCCCCAGGACTAACTTTGTTGGAAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240606164332_du19 AGTCAGTTCTTTCCAACAAAGTTAGTCCTGGG(+AAAAAA)GATCCAACTCAAGTCTCTAGACTTGGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240606164331_md6u GGCAGCCTCCACACCTGCTGTGCTTAGGACAG(+AAAAAA)GATCCTTTGATCACAAGGCAGAACCCTAACC NGG 10 8 17 8 24 0 100 5 10
    240606164330_un95 ACCGGGGTTAGGGTTCTGCCTTGTGATCAAAG(+AAAAAA)GATCCTGTCCTAAGCACAGCAGGTGTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606164329_w3d9 CCGCTAGCCAAGAAAACACTAGAGAGCTGCAG(+AAAAAA)GTACCTGCCACTTGGTCCTGGGAGGTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240606164327_bw8i GTGGCCTTTGACCTCCCAGGACCAAGTGGCAG(+AAAAAA)GTACCTGCAGCTCTCTAGTGTTTTCTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606164325_0msi CTCCCACTCCTTGGTCTGTGTGTTTCTCCAGG(+AAAAAA)GATCCCTGTCTGTGACTCTCCGCTGTCGGGG NGG 10 8 17 8 24 0 100 5 10
    240606164323_qx2t AACCCCCCCGACAGCGGAGAGTCACAGACAGG(+AAAAAA)GATCCCTGGAGAAACACACAGACCAAGGAGT NGG 10 8 17 8 24 0 100 5 10