Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164021_w4u5 AGTGTGAAATAAACAGGATAAAAAAGAAAATG(+AAAAAA)GTACCATGAGCAGGCAGAATGCTGCAATAAT NGG 10 8 17 8 24 0 100 5 10
    240606164020_1648 TATCAATTATTGCAGCATTCTGCCTGCTCATG(+AAAAAA)GTACCATTTTCTTTTTTATCCTGTTTATTTC NGG 10 8 17 8 24 0 100 5 10
    240606164018_ncjy GCAATCACATCGTGGCCAACCATCTGTAATGG(+AAAAAA)GATCCAATGCCCTCTTCAGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606164017_glqz GCTGTCTTCAGACATACCTGAAGAGGGCATTG(+AAAAAA)GATCCCATTACAGATGGTTGGCCACGATGTG NGG 10 8 17 8 24 0 100 5 10
    240606164015_vwdq TAGCATTCAAGCCATTCTTAGGAAGGACCAGG(+AAAAAA)GATCCTCTCTCATCTCTTGGGGTTGCCTTTC NGG 10 8 17 8 24 0 100 5 10
    240606164014_yu4q CCTAGGAAAGGCAACCCCAAGAGATGAGAGAG(+AAAAAA)GATCCCTGGTCCTTCCTAAGAATGGCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240606164013_em6j GCAGTAGATAATCAGGAAGATACAATGACTTG(+AAAAAA)GATCCTAGAACCACCTTCCTTTTCCTTTATG NGG 10 8 17 8 24 0 100 5 10
    240606164011_4hjw TCTTTCATAAAGGAAAAGGAAGGTGGTTCTAG(+AAAAAA)GATCCAAGTCATTGTATCTTCCTGATTATCT NGG 10 8 17 8 24 0 100 5 10
    240606164010_5nl3 TGTAGAAACTGGTGGTGTAGAAAGATACTGAG(+AAAAAA)GATCCAAGTTGTGTACTAGGACATGATTGAC NGG 10 8 17 8 24 0 100 5 10
    240606164009_vsd8 CTTGTGTCAATCATGTCCTAGTACACAACTTG(+AAAAAA)GATCCTCAGTATCTTTCTACACCACCAGTTT NGG 10 8 17 8 24 0 100 5 10