Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606164525_zkgi TTCTCCTTGTCTGTGGTGACCAGGGTGTAAAG(+AAAAAA)GTACCTGCTGCAGCTAACCTTGAACTTCACA NGG 10 8 17 8 24 0 100 5 10
    240606164524_566d GATAATGTGAAGTTCAAGGTTAGCTGCAGCAG(+AAAAAA)GTACCTTTACACCCTGGTCACCACAGACAAG NGG 10 8 17 8 24 0 100 5 10
    240606164523_pvvr GTGTTTTCTGTCCAAAACTCTGAAATAATAAG(+AAAAAA)GATCCCTTAGAGTATGGCAAAACCCCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606164521_p8sr ATTATGTTTGGGGGTTTTGCCATACTCTAAGG(+AAAAAA)GATCCTTATTATTTCAGAGTTTTGGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240606164520_cxwo GCCTAGTGCACATCTATGAGCCCATTATTCGG(+AAAAAA)GATCCAGAGGCAGAAGGATCAGAGTTCAAGG NGG 10 8 17 8 24 0 100 5 10
    240606164518_safv GATAACCTTGAACTCTGATCCTTCTGCCTCTG(+AAAAAA)GATCCCGAATAATGGGCTCATAGATGTGCAC NGG 10 8 17 8 24 0 100 5 10
    240606164517_kvzp AGCACCCTGTGAACAAAGAGCTGGTCACAGTG(+AAAAAA)GTACCCGAGTTTCTGTGACTGAGTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606164515_1yse GCTTTCAGACAGACTCAGTCACAGAAACTCGG(+AAAAAA)GTACCACTGTGACCAGCTCTTTGTTCACAGG NGG 10 8 17 8 24 0 100 5 10
    240606164513_oy3c TCTGCCCATTTAAAATGATAATGAACTATGGG(+AAAAAA)GTACCAAGCATAGTTAATATGTAAATACAAG NGG 10 8 17 8 24 0 100 5 10
    240606164512_ptcu ATGTACTTGTATTTACATATTAACTATGCTTG(+AAAAAA)GTACCCCATAGTTCATTATCATTTTAAATGG NGG 10 8 17 8 24 0 100 5 10